Regulation of hexose phosphate metabolism in Acetobacter xylinum

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Regulation of hexose phosphate metabolism in Acetobacter xylinum.

The metabolism of glucose and fructose was studied in resting succinate-grown cells of Acetobacter xylinum. From fructose only cellulose and CO(2) were formed by the cells, whereas from glucose, gluconate was formed much more rapidly than these two products. The molar ratio of sugar converted into cellulose to sugar converted into CO(2) was significantly greater than unity for both hexoses. The...

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Metabolism of Dicarboxylic Acids in Acetobacter Xylinum.

Benziman, Moshe (The Hebrew University of Jerusalem, Jerusalem, Israel), and A. Abeliovitz. Metabolism of dicarboxylic acids in Acetobacter xylinum. J. Bacteriol. 87:270-277. 1964.-During the oxidation of fumarate or l-malate by whole cells or extracts of Acetobacter xylinum grown on succinate, a keto acid accumulated in the medium in considerable amounts. This acid was identified as oxaloaceti...

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Metabolism of Hexose Phosphate Esters

Shortly after the discovery of insulin, it was shown by a number of investigators (Wigglesworth, Woodrow, Smith & Winter, 1923; Harrop & Benedict, 1924; Bollinger & Hartman, 1925) that there was a decrease in the inorganic phosphorus content of the blood in normal and diabetic animals following the injection ofinsulin. These observations first led to the hypothesis that insulin was concerned wi...

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Phosphorolytic Cleavage of Fructose-&phosphate by Fructose-6-phosphate Phosphoketolase from Acetobacter xylinum*

The synthesis of cellulose by washed suspensions of Acetobatter xylinum proceeds in the presence of iodoacetate at concentrations which completely inhibit the oxidation of glyceraldehyde-a-phosphate (1). Since glucose was the only carbon compound added, the possibility was considered that the energy required for the biosynthetic process may be derived from reactions linked to the pentose phosph...

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Encoding Cellulolytic Activity in Acetobacter xylinum

Recently, it was shown that a cellulose-negative mutant (Cell) ofAcetobacterxylinum ATCC 23769 carried an insertion of an indigenous transposable element (IS1031A) about 500 bp upstream of the bcs operon, required for cellulose synthesis. Here we show that Cell can be complemented by wild-type DNA covering the insertion point. Nucleotide sequencing of this region revealed the presence of two op...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1974

ISSN: 0264-6021

DOI: 10.1042/bj1380537